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Testing
Histopathology
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In order for the pathologist to provide the most meaningful interpretation, it is important to include a clinical history when submitting specimens for histopathology. Please include any relevant laboratory data, results of ancillary tests (e.g. radiographic or sonographic findings), provisional diagnosis (if any), as well as details about any current treatments. A gross description of the lesion is particularly useful, and should include details about:
Specimen Preparation
Punch biopsies, wedge biopsies and surgically excised tissues should be placed in
buffered 10% formalin in a ratio of 1:10 to ensure proper fixation. Small masses do not
need to be trimmed before submission. Larger masses may be trimmed, but only if the sectioning is complete, resulting in separate pieces (Partial sectioning results in distortion of tissues since they swell during fixation and cause difficulties in assessing the surgical margins).
It is best to trim very large specimens, submitting several representative samples, as samples more than . inch thick may not fix properly. Samples should be submitted in tightly sealed leak-proof containers to avoid spillage of formalin during transport.
Skin biopsies
3 to 5 biopsy samples from early or active lesions should be collected in order to capture any primary lesions and increase the chances of obtaining a specific diagnosis.
Eyes
Submit eyes whole but trim away the periocular tissues.
Bone
Biopsies of bone lesions must be deep enough in order to obtain a diagnostic sample.
Radiographs may be helpful in determining the depth at which to obtain a core biopsy.
These samples need to be decalcified prior to processing.
Heart
It is best to submit the entire heart submerged in formalin rather than a section of the heart since gross examination often yields more information than histopathology when pursuing a diagnosis of heart disease.
Postmortem samples
Necropsies should be performed as soon as possible after death to avoid autolytic changes
that may mask significant lesions. If there must be a delay, refrigeration of the carcass is preferable to freezing. Please include a clinical history and any gross necropsy findings.
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